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ExtFig6A_RACE_SLC7A5_R1_AI21_annotated.tiff

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https://figshare.com/articles/dataset/ExtFig6A_RACE_SLC7A5_R1_AI21_annotated_tiff/21810279
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HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters containing 99 bp, 51 bp, 27 bp or 15 bp insertions from the BCAP31 or SLC7A5 genes, and where indicated, with PR8 PA-X. RNA was extracted and used to run 5’ RACE with primers annealing to luciferase sequences. DNA bands were purified and sequenced to confirm their identities
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2023-01-19
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