AP-3-84 effects on gene expression in thioglycollate-induced, ID8 ascites or bone-marrow derived macrophages

Here we studied the effects of AP-3-84 treatment on gene expression in different types of macrophages: thioglycollate-induced macrophages, ascites macrophages from ID8 ovarian cancer-bearing mice, bone marrow-derived macrophages. We also studied whether AP-3-84 effects are mediated by p53 using bone marrow-derived macrophages from p53 KO mice. We found the dramatic changes in gene expression upon AP-3-84 treatment in all types of macrophages with the induction of major stress response programs and apoptosis signaling. Overall design: We isolated thioglycollate-induced macrophages, ascites macrophages from ID8 ovarian cancer-bearing mice. We also generated M0, M1 or M2-polarized macrophages using hematopoietic bone marrow stem cells from wild-type B6 or p53 KO B6 mice. We then treated these different types of macrophages with 10 uM AP-3-84 for 4 hours and analyzed gene expression changes by bulk RNA Sequencing.