Gene expression of 5-azacytidine-treated HCT116 cells. Gene expression of 5-azacytidine-treated HCT116 cells

DNA methylation plays an important role in regulating gene expression. In this study, a subpopulation with accelerated baseline motility (MG cells) or an immotile one (non-MG cells) from a colon cancer cell line (HCT116) were isolated, and gene expression levels of these cells with 5-azacytidine were measured by microarray analysis. Overall design: Five μM of 5-azacytidine or vehicle was treated into HCT116 cells for 48 hours and then gene expression was measured by human whole-genome microarray (Agilent Technology).