Polymeric-Based Theranostic Nanocarriers of Neuroprotective Drugs: Development, Imaging, and Bioanalysis

The following files include data presented in the manuscript "Polymeric-Based Theranostic Nanocarriers of Neuroprotective Drugs: Development, Imaging, and Bioanalysis" written by Krzysztof Szczepanowicz, Magdalena Procner, Marta Szczęch, Natalia Łopuszyńska, Danuta Jantas, Magdalena Regulska, Monika Leśkiewicz, Krzysztof Jasiński, Kamil Stachurski, Lilianna Szyk-Warszyńska, Adam Roman, Władysław Lasoń, Władysław P. Węglarz, and Piotr Warszyński.Inefficient delivery of neuroprotective drugs to their target sites remains a major impediment in the treatment of neurodegenerative disorders. Therefore, our research was focused on a new strategy for the preparation of polymeric-based theranostic nanocarriers of neuroprotective drugs. Polymeric theranostic nanocarriers of calcineurin inhibitors, Cyclosporin A (CsA) and Tacrolimus (FK506), as potential neuroprotective agents, were prepared via the self-emulsification solvent evaporation (SESE) method with the combination of a layer-by-layer technique. For magnetic resonance imaging, gadolinium-labeled poly-L-lysine (PLLGd) was used, while for optical imaging, rhodamine-labeled poly-L-lysine (PLL-ROD) was used. The dataset includes experimental data corresponding to the figures and tables presented in the publication. It contains characterization of the physicochemical properties of nanocarriers (such as size distribution, absorption, and emission spectra), as well as MRI data for nanoparticle relaxivity (r₁, r₂) measurements.It also comprises processed results from human neuroblastoma cells, primary neuronal cell cultures, and brain microvascular endothelial cells, including cytotoxicity (LDH assay), cell viability (WST-1 and MTT assays), cellular uptake, transepithelial electrical resistance measurements, and the profile of nanocarrier transfer across an artificial blood-brain barrier (BBB) model.