Evaluation of linkage mapping results by ANOVA.

GLM analysis was used to evaluate whether SNP markers within candidate Al tolerance genes explained significance variance for Al tolerance observed in F2 populations. Gene action was modeled as either additive (“add”) or dominant (“dom”) based on allelic means. The variance explained by each significant SNP is reported. As both SAHH and ZmASL were significantly associated with Al tolerance for the B73×CML247 population, a summary model is reported. DF: Degrees of Freedom; SS: Sum of Squares; F: F ratio; P: P value.