Substrate specificity of rPSTG.
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apNP, p-nitrophenyl.
bA linear relationship between initial velocity (v) and substrate concentration [S] was obtained in the range of [S] examined (up to 10 mM), suggesting that the Km value for the substrate should be significantly higher than 10 mM, because the enzyme-catalyzed reactions proceeds with first-order kinetics under the conditions of [S]<<Km as described by v = (kcat/Km)[S], where kcat/Km is the first-order rate constant. Thus, the ratio kcat/Km could be determined from slope of v versus [S] plots.
nd, not determined.
创建时间:
2013-04-10



