Identification of proteins that presented a significant decrease in their expression content.

a) Spots match ID number obtained from ImageMaster Platinum;
b) Name of the identified protein;
c) Uniprot identification code;
d) Experimentally predicted and expected isoelectric point (pI);
e) Experimentally predicted and expected molecular weight (Mr, in kDa);
f) Number of identified peptides by MS;
g) Percentage of the protein sequence covered by identified peptides;
h) Normalized data from R0 represented by mean values of each condition divided by R30 value;
i) Fold represents the maximum spot intensity mean value of the conditions divided by the smallest value;
j) One-way ANOVA (P<0.01) obtained from spot analysis;
k) Biological functions according to NCBI, UniProt, and Gene Ontology databases;
l) Biological activity and/or immunological application described in other studies: [22] Tull et al., 2010; [23] Oliveira et al., 2006; [24] Daifalla et al., 2011; [25] Iyer et al., 2008; [26] Hunger-Glaser et al., 1999; [27] Werbovetz et al., 1999; [28] Feng et al., 2011; [29] Niemirowicz et al., 2007; [30] Hunger-Glaser et al., 1997; [31] Alcolea et al., 2009; [32] Bhaskar et al., 2012; [33] Khanra et al., 2012; [34] Berberich et al., 2003; [35] Moore et al., 1996; [36] Swenerton et al., 2011; [37] Hummadi et al., 2006; [38] Martins et al., 2006; [39] Burns et al., 1993; [40] Kushawaha et al., 2011; [41] Misra et al., 2005; [42] Bringaud et al., 1995; [43] Eggleson et al., 1999; [44] Mureev et al., 2007; [45] Steiner et al., 2007; [46] Martínez-Rodríguez et al., 2012; [47] Drummelsmith et al., 2004; [48] Achour et al., 2002; [49] Buda et al., 2013; [50] Alcolea et al., 2011; [51] Martín et al., 2009; [52] Silva et al., 2012. The proteins were identified through the data included in the NCBI database (dated June 2012) for Leishmania spp.