NRCs from solanaceous plants displayed diverse compatibility with sensor NLRs.

(A) Workflow of cell death assays testing the ability of NRC to function with different sensor NLRs. NRCs were co-expressed with sensor NLRs and the corresponding effectors in nrc2/3/4_KO N. benthamiana. NRC variants were co-expressed with (B) Rx/CP, (C) Sw5b/NSm, (D) Gpa2/RBP1, (E) Pto/AvrPto, or (F) R1/AVR1 to analyze their ability to work with sensor NLRs. Cell death phenotypes were recorded at 6 dpi. The dot plots represent cell death intensity quantified using UVP ChemStudio PLUS. The line in the boxplots represents the medium, the box edges represent the 25th and 75th percentiles, and the whiskers extend to the most extreme data points no more than 1.5x of the interquartile range. Statistical differences between the negative control (EV) and tested groups were examined by paired Wilcoxon signed rank test (* = p < 0.0001).
(JPF)