ST2+ T-regulatory Cells in Renal Inflammation and Fibrosis following Ischemic Kidney Injury

The role of IL-33 and its receptor ST2 in kidney injury is not well understood. This study demonstrates the importance of ST2 expression on the T-regulatory cells (Tregs) for the resolution of renal injury by suppressing inflammation and fibrosis. Treg-specific deletion of ST2, detrimentally impacted late outcomes of renal injury worsening the impairment of kidney function, inflammation, and fibrosis. Overall design: To delineate the role of kidney-specific ST2+ Tregs, we utilized single-cell RNA sequencing platform. Post unilateral IRI CD4 T cells were sorted from digested kidneys of Il1rl1fl/fl and Il1rl1fl/fl Foxp3YFP-Cre mice respectively using flow cytometry. The samples from Il1rl1fl/fl mice, IRIWT1 (10,146 cells) and IRIWT2 (9,573 cells) were merged as IRIWT, and Il1rl1fl/fl Foxp3YFP-Cre mice, IRIKO1 (4,580 cells), IRIKO2 (10,899 cells) and IRIKO3 (5,580 cells) were merged as IRIKO respectively.