Differences in RAD51 transcriptional response and cell cycle dynamics reveal varying sensitivity to DNA damage among Arabidopsis thaliana root cell types

Throughout their lifecycle, plants are subjected to DNA damage from various sources, both environmental and endogenous. Investigating the mechanisms of the DNA damage response (DDR) is essential to unravel how plants adapt to the changing environment, which can induce varying amounts of DNA damage. Using a combination of whole-mount single-molecule RNA fluorescence in situ hybridization (WM-smFISH) and plant cell cycle reporter lines, we investigated the transcriptional activation of a key homologous recombination (HR) gene, RAD51, in response to increasing amounts of DNA damage in Arabidopsis thaliana roots. The results uncover consistent variations in RAD51 transcriptional response and cell cycle arrest among distinct cell types and developmental zones. Furthermore, we demonstrate that DNA damage induced by genotoxic stress results in RAD51 transcription throughout the whole cell cycle, dissociating its traditional link with S/G2 phases. This work advances the current comprehension of..., , , # Data from: Differences in RAD51 transcriptional response and cell cycle dynamics reveal varying sensitivity to DNA damage among Arabidopsis thaliana root cell types

[https://doi.org/10.5061/dryad.9w0vt4bq3](https://doi.org/10.5061/dryad.9w0vt4bq3)

Here we provide all the raw data images for the manuscript: Kutashev et al. 2024 *New Phytologist* (DOI: 10.1111/NPH.19875).

## Description of the data and file structure

Files are organized according to figure numbers as described in the manuscript (DOI: 10.1111/NPH.19875). Please refer to the methods section of the manuscript for details on the imaging settings.

Figure 1 - Raw microscopy images for Immunofluorescence (anti-gammaH2AX) and single-molecule RNA FISH (detection of RAD51 transcripts).

Figure 2 - Raw microscopy images for wholemount single molecule RNA FISH (detection of RAD51 transcripts).

Figure 3 - Raw microscopy images for wholemount single molecule RNA FISH using RAD51-GFP line (detection of RAD51 protein and RNAs).

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