Data presented in Brinkmann et al., 2022, Ecotoxicol. Environ. Saf.

These files include all data presented in:

Brinkmann et al., 2022. Microbiota-dependent TLR2 signaling reduces silver nanoparticle toxicity to zebrafish larvae. Ecotoxicology and Environmental Safety.

GFAAS_ICP_MS_(..)
Measurement of total Ag concentration in zebrafish larvae exposed to nAg from 3-5 dpf (Fig. 2):
* Sample: The tissue that was sampled, either the complete larvae or extracted intestines.
* Time: Exposure time (hours).
* ppbAg: Total concentration of Ag (ppb) that was measured.
* AgaroseVolume: Volume (μL) of agarose in the digestion mixture.
* AquaRegiaVolume: Volume (μL) of aqua regia in the digestion mixture.
* TotalDigestionVolume: Total digestion volume (μL).
* ngAg_larva: Total mass of Ag (ng) that was measured per larva.
* Method: Detection method, either GFAAS or ICP_MS.
The first row specifies the limit of detection.

il1beta_eGFP_(...)
Three folders comprising fluorescence microscopy images (TIFF format) of il1beta:eGFP reporter zebrafish larvae at 5 dpf (Fig. 3):
* (...)_replicates1_20200226: images for the first experimental replicate
* (...)_replicates2_20200304: images for the second experimental replicate
* (...)_replicates3_20200318: images for the third experimental replicate
For each of the replicates, the following images were acquired:
* nAg_GFP: GFP signal for larvae exposed to nAg.
* nAg_trans: transmitted light images for larvae exposed to nAg.
* Agion_GFP: GFP signal for larvae exposed to silver ions.
* Agion_trans: transmitted light images for larvae exposed to silver ions.
* control_GFP: GFP signal for control larvae that had not been exposed to silver ions or nAg
* control_trans: transmitted light images for control larvae that had not been exposed to silver ions or nAg.

DoseResponses_(...)
Mortality data of zebrafish larvae with loss-of-function mutations for TLR2, MyD88 and TIRAP, and their wildtype siblings, exposed to nAg from 3-5 dpf (Fig. 4-5):
* Concentration: Nominal exposure concentration (mg nAg/L)
* Condition: Microbial condition of the zebrafish larvae, either colonized or germ-free.
* Hours: Exposure time (hours).
* Mortality: Percentage of larvae that had died.
* Date: The date at which the mortality was scored.
* Gene: Specifies the concerning genes for loss-of-function mutations, either TLR2, MyD88 or TIRAP.
* Genotype: Specifies the genotype concerning the loss-of-function mutations, either mutant (-/-) or WT sibling (+/+).
* Replicate: The replicate that was scored, either 1, 2 or 3.