Investigating Higher-Order Chromosomal Structures in Saccharomyces cerevisiae Meiosis Using CheC-PLS

We developed CheC-PLS, a proximity-labeling technique that indelibly marks and then decodes protein-associated sites. CheC-PLS tethers dam methyltransferase to a protein of interest, followed by Nanopore sequencing to identify methylated bases - indicative of in vivo proximity - along reads >100kb. Data reveals cohesin translocation on DNA and, by anchoring reads onto unique regions, we define the internal organization of the ribosomal DNA locus.