B-cell lymphoma, NOS - Male, 50 - Cell image [3300730069481171]

This is the histopathological image of HEMATOPOIETIC AND RETICULOENDOTHELIAL SYSTEMS cell sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format. The detailed information about the patient, sample, and diagnosis are as follows: Patient:Age: 50Clinical description: The material was taken from the region of the left anterior superior iliac spine. The patient is undergoing a diagnosis of the lymphoproliferative process. Immunophenotyping of blood and marrow suggests marginal zone lymphoma.Gender: MaleDiagnosis:Classification: ICD-O_3.2Classification code: MALIGNANT LYMPHOMAS NOS OR DIFFUSEDiagnosis: B-cell lymphoma, NOSResult of the histopathological examination: Trepanobioptate in two fragments of a total length of 23 mm. Cellularity is approximately 90%. The bone marrow shows infiltrates of medium to large B lymphocytes with phenotype: CD45(+), CD20(+), Bcl2(+), CD43(+/-), MUM1(+), c-myc(+/-, in about 30% of cells). Reactions with CD10, Bcl6, CD5, Cyclin D1, SOX11, Annexin A1, DBA44, Tdt, CD34, CD23 and MPO are negative. MIB1/Ki67 60%. The infiltrates described above represent about 80% of all cells. In residually preserved normal hematopoietic tissue, E: M ratio =1:2. Granulocytic lineage (MPO+) with rejuvenation. Blastic cells with CD34 expression represent approximately 1% of all cells. Red cell lineage (CD71+) depleted. Quite numerous megakaryocytes (CD61+). Focal reticulin fibers (MF-1). The microscopic picture supports the diagnosis of B-cell lymphoma of high malignancy. Together with the phenotype determined cytometrically and the clinical presentation (splenomegaly), this is most likely a progression/transformation of splenic lymphoma (SMZL).Sample:Material: FFPECollecting method: Bone marrow aspirationTopography: HEMATOPOIETIC AND RETICULOENDOTHELIAL SYSTEMSOrgan: HEMATOPOIETIC AND RETICULOENDOTHELIAL SYSTEMSTissue: Bone marrowType of staining: positive/IHCStaining: Not applicableAntibody: Ki-67Technology:Equipment: Pannoramic 250 3DHistechLens: 20xOrganization:Source: Medical University Gdańsk