Identification of competing endogenous RNA (ceRNA) and micro-RNA profiles and regulatory networks in 4-nonylphenol-induced impairment of sertoli cells II

Purpose: To uncover the mechanism of 4-nonylphenol-induced impairment of sertoli cells, we used RNA sequencing technology to compare the small RNA expression differences between the control groups and the 4-nonylphenol treated groups. Sertoli cells small RNA profiles of the control groups and the 4-nonylphenol treated groups were generated by deep sequencing, in triplicate. The library quality was determined using a Bioanalyzer 2100 (Agilent). The Illumina BGISEQ platform was used for small RNA sequencing. The quality of RNA-seq reads was examined using FastQC.本研究旨在阐明4-壬基酚（4-nonylphenol）诱导支持细胞（Sertoli cells）损伤的分子机制，采用RNA测序（RNA sequencing）技术对比对照组与4-壬基酚处理组的小RNA（small RNA）表达差异。本研究通过深度测序（deep sequencing）构建了三组生物学重复的对照组与4-壬基酚处理组支持细胞的小RNA表达谱。文库质量通过安捷伦（Agilent）Bioanalyzer 2100生物分析仪进行检测。小RNA测序工作采用Illumina BGISEQ测序平台完成。使用FastQC对RNA测序reads的质量进行质控分析。