Umbilical cord blood methylome analysis of IVF neonates that underwent embryo culture in G5 or HTF medium

Follow-up study of IVF neonates who underwent embryo culture in G5 (Vitrolife) or HTF (Lonza) medium. Genome-wide DNA methylation profiling of IVF neonates (umbilical cord blood samples) who had undergone embryo culture in G5 medium (Vitrolife) or HTF medium (Lonza). The EPIC array was used to profile the methylome at approximately 850,000 CpG sites across the human genome. Bisulphite converted DNA from umbilical cord blood samples from IVF neonates that had undergone embryo culture in G5 (n = 59 ) or HTF (n = 47) culture medium, were hybridized to the Illumina EPIC array. Data processing was conducted in R using the RnBeads package. Processing consisted of SWAN normalization, and site/sample removal based on greedycut (detection p-value threshold 0.05), SNP proximity, position on the sex chromosomes, missing values in >5% of samples and sites not in a CpG context.