Next Generation Sequencing Analysis of livers from 2-month WT/12-month WT and 2-month HKO/12-month nontumor/12month- tumor.

SRSF2 flanked with flox was specifically depleted in the livers by Alb-Cre(HKO),and took the intact ones as contorl(WT).Near 70% of the HKO livers spontaneously developed malignant tumors(took the tumor areas as tumor while the tumor-adjacent as nontumor).Then total RNAs of 2-month WT/HKO and 12-month WT/nontumor/tumor were isolated and performed the next generation sequencing. We profiled the gene expression regulated by Cre-loxp-mediated knockout system. Overall design: mRNA profiles of w8-WT80,w8-HKO82,M13-WT44,M13-HKO24,M13-tumor24 liver samples were generated by deep sequencing.