Kinase assay - GRPR activation
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https://data.mendeley.com/datasets/nwkpyr2nmh
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资源简介：
A kinase assay was carried out using the STK pamgene kinase assay to understand what was the signaling induced by the activation of GRPR. 1057 mouse melanoma cells were stimulated for 15 minutes with 10 nM GRP and/or 1µM RC-3095 in F12 0.5% FBS. Proteins were extracted with M-Per buffer supplemented with Halt protease Inhibitor cocktail (Thermo Scientific #78430) and Halt phosphatase inhibitor cocktail (Thermo Fisher #78442) and immediatly snap-frozen. 1 µg of proteins were analysed using STK chips and ran according to the manufacturer recommendation.
Four biological replicates were generated
Groups:
- MOCK = 0.1% DMSO
- GRP = 10nM GRP in 0.1% DMSO
- RC = 1µM RC-3095 in 0.1% DMSO
- MOCK = 10nM GRP + 1µM RC-3095 in 0.1% DMSO
创建时间：
2025-04-17
