Hallmarks of CD8 T cell dysfunction are established within hours of tumor antigen encounter

RNA-SEQ analysis of antigen-specific CD8 T cells sorted from murine liver tumors and from the spleens of Listeria-infected mice at different early timepoints following adoptive transfer. We sequenced naive antigen-specific CD8 T cells from spleens for comparison. CD8+CD90.1+ TAG-specific T cells (1) sorted from murine liver tumors (TAG-expressing tumors) at 6, 12, and 24 hours or (2) sroted from spleens of Listeria-TAG infected mice at 6, 12, and 24 hours of antigen exposure or (3) sorted from spleens of transgenic TCRTAG mice, and RNA-SEQ was performed.