Cell cycle regulator MYBL2 is a distinct vulnerability in acute myeloid leukemia

We were interested in transcriptional changes after depletion of MYBL2 in acute myeloid leukemia. Therefore, we utilized THP-1 and NOMO-1 as models, and analyzed global alterations of gene expression. Overall design: Transcriptomic changes in THP-1 and NOMO-1 after CRISPRi-based MYBL2 knockdown. RNA-Sequencing from THP-1/NOMO-1 cells stably expressing dCas9-KRAB-GFP after transduction with either a sgRNA against a non-targeting luciferase (luc) or MYBL2. Experiment was performed in biologically independent triplates.