Chloroplast motion under dim light conditions

<em>Elodea densa </em>was kept in an aquatic culture. Ambient light conditions were applied 1h before image acquisition. <br> Imaging preparation: a low-light adapted leaf was detached and placed between two glass slides.<br> Lower cell layer is displayed. <br> Microscopy: Bright field microscopy was performed with a Nikon TI2 microscope using a halogen light source and a red-light 620nm cut-on wavelength filter at low light intensities of 7.2 W/cm^2 in combination with a Photometrics BSI Express sCMOS camera with high quantum yield to enable imaging every 1s with a $63\times$ water-immersion objective (NA=1.2) and pixel resolution of 0.1µm/px. <br> <br> Description:<br> Chloroplast.zip:<br> Contains ome.tiff files for 3600 frames of chloroplast motion (total duration 1h)<br> The dense chloroplasts (dark circular objects) inside the plant cells move by actin-mediated propulsion mechanism. <br> <br> 211013_CP_rawLabelMeasures.csv:<br> Analysis of segmented data (3600 frames) <br> <br> 211013_CP_tracks_vel.csv:<br> Trajectories (with velocities) constructed by trackpy from center-of-mass positions of the 211013_CP_rawLabelMeasures.csv. <br> Velocities where calculated using the first derivative of 3rd order Savitzky-Golay filtered data (Kernel width 11s). <br> <br>