Insulin Resistance Increases TNBC Aggressiveness and Brain Metastasis via Adipocyte-derived Exosomes

This dataset includes mRNA sequencing data obtained from 4T1 cells, either from in vitro cell culture, or clonogenic assay from brain, or primary tumor treated with exosomes from insulin sensitive (IS) or induslin resistant (IR) adipocytes; and 4T1 cells cultured in vitro and treated with miR-145a-3p. Exosomal small RNA from IS and IR aidpocytes derived exsomes were also included. RNA was extracted and sequenced to investigate differential gene expression and associated biological pathways. The aim of the study is to elucidate how insulin resistance in adipocytes influences gene expression, cancer aggressiveness and metastasis. Data include raw and processed mRNA sequencing files from replicates for each condition. Overall design: Cells were treated with exosomes derived from IS or IR adipocytes. Total RNA was extracted using the RNeasy Plus Mini Kit (QIAGEN, Cat. No. 74136) following the manufacturer's instructions. Sequencing libraries were prepared and sequenced using the Illumina platform to produce paired-end reads. Data were processed using the nf-core RNA-seq pipeline, and differential expression was analyzed using DESeq2. Low-expressed genes (fewer than 3 reads per million in at least 3 samples) were filtered out, and data were normalized for library size differences. Differentially expressed genes (DEGs) were analyzed for pathway enrichment using Gene Ontology (GO) and further correlated with survival outcomes in breast cancer patients from The Cancer Genome Atlas (TCGA). Survival analysis was conducted using Cox proportional hazards models.