Effect of the microbiome on pathogen susceptibility across four Drosophilidae species

The microbiota has been shown to play an important role in host susceptibility to infections in some hosts. However, less is known about whether microbiota-mediated effects are consistent across host species, as our understanding of such interactions may be affected by publication bias. Following on from a large study of 36 species of Drosophilidae challenged with four bacterial pathogens, we identified two candidate host species that might have protective microbiome based on low susceptibility and high abundance of the culturable microbiota; we selected two other host species for comparison. We tested whether germ-reduced flies, and flies with a natural or re-constituted microbiome, varied in their susceptibility to systemic infection with two bacterial pathogens (Providencia rettgeri and Staphylococcus aureus) and one viral pathogen (Drosophila C Virus).