microRNA responses of LPS and IL-10 stimulated primary human monocyte-derived macrophages over a short time-course. microRNA responses of LPS and IL-10 stimulated primary human monocyte-derived macrophages over a short time-course

Lipopolysaccharide stimulated primary human monocyte-derived macrophages at 2, 4 and 8hrs, IL-10 at 4hrs, IL-10+LPS at 4hrs Overall design: Dual label approach with each test sample was labelled with Hy3, and a “common reference” sample (obtained by combining equal quantities of RNA from each of the six experimental data points) was labelled with Hy5. Pooled donor approach: each test sample was pooled from 4 independent experiments with different human donors