Expression Data from Arabidopsis ULT1 and CLF Mutants

ULT1 and CLF function antagonistically as epigenetic regulators of gene expression in Arabidopsis. We sought to identity their global downstream target genes at two stages of plant development and determine their common targets. We used microarrays to determine the global gene expression programs regulated by ULT1 and FLC during vegetative and reproductive development and compared the overlap among target genes between the two proteins. Arabidopsis shoot apices from wild-type Ler, ult1-3 Ler, 35S:ULT1 Ler, and clf-2 Ler plants were collected at the vegetative and reproductive stages for RNA extraction and hybridization on Affymetrix microarrays.