MNase and HpaII sensitivity assays in MCF7 breast cancer cells. MNase and HpaII sensitivity assays in MCF7 breast cancer cells

DNA are packaged into nucleosomes and chromatin. We performed incomplete MNase digestion of chromatin to identify "nucleosome-free" regions that may indicate active promoters and regulatory regions. Additionally, HpaII digestion was performed which cleaves CCGG sites when the internal C remains unmodified. The hypomethylation state and nuclease sensitivity of the chromatin are indicators of transcription regulatory regions.