Effects of intrauterine hyperglycemia on offspring development in gestational diabetes mellitus based on placental transcriptomics

Gestational diabetes (GDM) refers to diabetes diagnosed as diabetes in the second or third trimester of pregnancy, but not obvious before pregnancy. It is one of the most common complications of pregnancy, which can lead to perinatal asphyxia, neonatal hypoglycemia, macrosomia, cardiovascular abnormalities, obesity and other short-term and long-term adverse outcomes of the offspring. This study explores the differential expression of transcriptome between GDM and non GDM placenta, and explores the impact of subtle metabolic changes in the placenta on fetal growth and development during GDM in pregnant women. Overall design: In this study, 16 samples of gestational diabetes (GDM, n=8) and non gestational diabetes (n=8) were analyzed by transcriptome sequencing (RNA seq). Using | log2Fold Change |>=2 and FDR<0.05 as the standard for screening differentially expressed genes (DEGs), GO functional annotation, KEGG pathway analysis, and protein-protein interaction network (PPI) analysis were performed on the selected differentially expressed genes (DEGs).