The 350 bp core region of the +45 element insulates transgenic constructs and improves tissue-specific expression from the Scl promoter.
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https://figshare.com/articles/dataset/_The_350_bp_core_region_of_the_45_element_insulates_transgenic_constructs_and_improves_tissue_specific_expression_from_the_Scl_promoter_/344447
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The total number of F0 transgenic embryos obtained with each construct was determined by PCR using specific primers. Upon ß-galactosidase staining, transgenic embryos were visually inspected and scored as either LacZ+ or LacZ- and the percentage of LacZ+ embryos calculated.
*p<0.05 compared to SV/LacZ. The expression pattern of Scl/LacZ and 350/Scl/LacZ/350 transgenic embryos is considered ectopic if beyond the mid and hind brain, the expected expression pattern for the Scl promoter alone.
**p<0.01 compared to Scl/LacZ.
创建时间：
2012-03-01
