Single-cell RNA sequencing of lymphoblastoid cell lines (LCL) from two human populations.. Homo sapiens

Lymphoblastoid cell lines (LCLs) are the preferred choice of storage for patients' genetic material. Through the analysis of RNAs from LCLs, several mutations related with various diseases have identified. LCLs encompasses a wide range of metabolic pathways that are specific to individuals where the cells originated, making LCLs suitable for molecular and functional studies. Large-scale sequencing efforts have documented extensive genetic variation within the human populations. However, with the development of new methods to sequence the RNA at a single-cell level, our understanding of the origins, global distribution, and functional consequences of this variation now more far from complete than before. To better understand the spectrum of gene expression variation, we have sequenced two LCL samples (GM18502 and GM12878) derived from female donors of two human populations (YRI and CEU).