RIP-seq for Prrc2a in mouse brain sample
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https://www.ncbi.nlm.nih.gov/sra/SRP110524
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RNA was isolated from widetype C57BL/6J using the TRIzol (Invitrogen) reagent by following the company manual. For all samples the RNA integrity was checked using an Agilent Bioanalyzer 2100. All samples showed a RIN (RNA integrity number) of higher than 9. Approximately 2.5 µg of total RNA was then used for library preparation using a TruSeq⢠RNA Sample Prep Kit v2 (Illumina, San Diego, CA, USA) according to the manufacturer's protocol.The libraries were sequenced using HiSeq2500 or 3000 (Illumina) in paired-read mode, creating reads with a length of 125 or 101 bp. Sequencing chemistry v2 (Illumina) was used. Overall design: Examination of Prrc2a binding targets in wild type mouse brain
创建时间：
2019-09-23
