Mus musculus domesticus strain:C57BL/6 Transcriptome or Gene expression. Mus musculus domesticus strain:C57BL/6

We used RNA-seq to obtain a high-resolution transcriptome data set of murine osteoblast differentiation in vitro. Primary calvaria osteoblasts were harvested from newborn C57BL/6 wild-type mice (P0-P4). Cells were seeded on 6-well plates in Alpha-Mem containing 10 % fetal calf serum as well as Pen/Strep and 2 mM ultra-glutamine. At confluence as defined here as day 0, cells were harvested and on the other plates medium was supplemented with 50 µM L-ascorbate-2-phosphate and 10 mM beta-glycerophosphate to promote osteoblast differentiation. Cells were harvested at four distinct time points: within proliferation (day 0), during maturation (day 3), terminal differentiation (day 6), and at the onset of mineralization (day 12).