Stomatal patterning is differently regulated in adaxial and abaxial epidermis in Arabidopsis

Stomatal pores in leaves mediate CO2 uptake into the plant and water loss via transpiration. Most plants are hypostomatous with stomata present only in the lower leaf surface (abaxial epidermis). Many herbs, including the model plant Arabidopsis thaliana, have substantial numbers of stomata also on the upper (adaxial) leaf surface. Studies of stomatal development have mostly focused on abaxial stomata and very little is known of adaxial stomatal formation. We addressed the role of leaf number in determination of stomatal density and stomatal ratio, and studied adaxial and abaxial stomatal patterns in mutants deficient in known abaxial stomatal development regulators. We found that stomatal density in some genetic backgrounds varies between different fully expanded leaves and recommend using defined leaves for analyses of stomatal patterning. Our results indicate that stomatal development is at least partly independently regulated in adaxial and abaxial epidermis, as i) plants deficient ..., , , # Data from: Stomatal patterning is differently regulated in adaxial and abaxial epidermis in Arabidopsis

[https://doi.org/10.5061/dryad.s4mw6m9g0](https://doi.org/10.5061/dryad.s4mw6m9g0)

## Description of the data and file structure

The data are from two experiments:

First experiment: The data includes adaxial and abaxial stomatal density and stomatal ratio determined on leaves 6 and 8 of 7-week-old Arabidopsis (*Arabidopsis thaliana*) plants and on leaves 10 and 12 of 8-week-old Arabidopsis plants via dental silicone and nail varnish impressions and brightfield microscopy.

Second experiment: The data includes adaxial and abaxial stomatal density, size and index, total stomatal density from leaf 8 of 6-week-old Arabidopsis plants collected via dental silicone and nail varnish impressions and brightfield microscopy. In addition, projected rosette area was determined from the same plants a 4 weeks of age.

### Files and variables

#### File: Supplementary\_Data.xlsx

**Description:...