Additional file 11: of Comparative analyses of glycerotoxin expression unveil a novel structural organization of the bloodworm venom system

sheet a Summary statistics of filtering steps (using cutadapt v.1.8.1 and ConDeTri v.2.2) performed on raw Illumina sequencing data of three different tissue types (putative venom glands, pharyngeal lobes, and posterior body wall) analyzed in G. tridactyla. Transcriptome libraries were constructed for a single individual (SR21, putative venom glands; SR23, pharyngeal lobes; and SR25, posterior body wall), and based on pooled samples comprising RNA of three individuals (SR22, putative venom glands; SR24, pharyngeal lobes; and SR26, posterior body wall). Abbreviations: PE, paired-end reads; SR, single reads. sheet b Summary statistics of IDBA-tran v.1.1.1 assemblies performed on processed Illumina sequencing data originating from G. tridactyla. Assemblies were done for three different tissue types of a single individual (SR21, putative venom glands; SR23, pharyngeal lobes; and SR25, posterior body wall), and for Illumina sequencing data of three pooled individuals (SR22, putative venom glands; SR24, pharyngeal lobes; and SR26, posterior body wall). In addition, the filtered reads of single and pooled libraries were merged (SR21 + SR22, putative venom glands; SR23 + SR24, pharyngeal lobes; SR25 + SR26, posterior body wall) to perform comparative expression studies by mapping them against GLTx reference sequences. (XLSX 17 kb)