2D Measurement of Retinal Pigment Epithelium Function Using Quantitative Bright-Field Microscopy

Induced Retinal Pigment Epithelial (iRPE) implants were developed from two healthy donors (Healthy-1 and Healthy-2). Live iRPE were imaged using quantitative bright-field absorbance microscopy (QBAM) every week during the in vitro maturation process. The data are organized by the culture plate, imaging date and time, color filter used to capture the images, and finally images that include the well ID and grid position in the name of the image. Both Healthy-1 and Healthy-2 iRPE were cultured in 12-well plates as outlined in Figure 1, with only half of the 12-well plate containing cells (green circles, Figure 1). The Blank Well (blue circles) was filled with culture medium but contained no cells and was used for benchmarking and calibration protocols that are part of the QBAM process. The grid in each well indicates that a 4x3 grid of overlapping images (~10-15% overlap) were captured for each well. One unique characteristic of this dataset is that each plate contains iRPE treated maturation inhibitors (negative control, HPI4), maturation promotors (positive control, Aphidicolin), or neither. The imaging parameters and functional data collected for each dataset for Healthy-1 and Healthy-2 were different, and the details of data collection and contents are included in the DataSummary.txt file included in each subfolder.