Characterization of pdgfrb reporter expressing cells from the adult zebrafish heart ventricles by single cell RNA sequencing

Platelet-derived growth factor (PDGF) signaling regulates perivascular cell or mural cell association with the blood-vessel endothelial cells. Mural cells express the receptor of the signaling, pdgfrb. Besides mural cells the outer epithelial layer of the heart, epicardium and epicardial derived cells express pdgfrb. In this dataset we characterized pdgfrb expressing cells in adult zebrafish heart ventricles in AB wildtype fish, pdgfrb loss-of-function mutant fish. Since pdgfrb is activated in injured zebrafish heart, we aslo compared pdgfrb expressing cells in injured (amputated, 7 days post amputation) heart ventricle with similar cells in uninjured hearts. Overall design: We FACS sorted Tg(pdgfrb:EGFP) reporter expressing cells from the adult heart ventricles of the wildtype, pdgfrb mutant Zebrafish. We also FACS sorted these cells from adult uninjured and injured (7 days post amputation) heart ventricles and performed single cell RNA sequencing. All zebrafish used here were of AB strain.