Single-cell level gene expression profiles of enteroendocrine cell lineages, control guts and guts with Xrp1 overexpressed in EEs

Differentiated cells exhibit cell plasticity, where they dedifferentiate to stem cells or trans-differentiate into cells of another lineage, when necessary. We studied the cell plasticity of secretory enternendocrine cells (EEs) in the fruit fly Drosophila melanogaster. We resolved single-cell transcriptomics of EEs and cells derived from EEs. Our scRNA-seq data of EE lineages have characterized intermediate cell types derived from EEs. We also specifically focused on a gene called Xrp1 and examined potential changes of all epithelial cell types by EE-specific Xrp1 overexpression (O/E). Our scRNA-seq data of Xrp1 O/E EEs have provided evidence on changes in gene expression profiles of each cell type by Xrp1 O/E in EEs. Overall design: Flies had EEs lineage traced from adulthood to 7 days of age. Cells from their midguts were collected and isolated by Fluorescence Activated Cell Sorting. Flies with Xrp1 overexpressed in EEs had their midgut cells collected. Cells were proceeded to 10X Genomics scRNA-seq.