Construction of cynomolgus monkey cell atlas by scATAC seq and scRNA seq

In this study, we tried to create a cellular transcriptome map of cynomolgus monkeys at the single-cell level. The cell landscape covers major organs including adipose, aorta, bladder, breast, colon, heart, kidney, liver, lung, muscle, spleen, stomach, testis, tongue, trachea and uterus. We also perform an integrative analysis of scRNA-seq and scATAC-seq of several organs to define transcriptional and epigenetic changes in cynomolgus monkey. Importantly, we have compared the differences in cell maps of human, mouse and monkey, we hope to provide sufficient information about primates for subsequent preclinical experiments.本研究旨在构建食蟹猴（cynomolgus monkey）的单细胞水平细胞转录组图谱。该细胞图谱涵盖脂肪组织、主动脉、膀胱、乳腺、结肠、心脏、肾脏、肝脏、肺脏、肌肉、脾脏、胃、睾丸、舌、气管及子宫等主要器官。本研究还对多个器官的单细胞RNA测序（single-cell RNA sequencing, scRNA-seq）与单细胞转座酶可及性测序（single-cell assay for transposase-accessible chromatin sequencing, scATAC-seq）进行整合分析，以解析食蟹猴的转录组与表观基因组变化。尤为关键的是，本研究已对比人类、小鼠与食蟹猴的细胞图谱差异，以期为后续临床前实验提供充足的灵长类动物相关研究数据。