Data from: An engineered prodrug selectively suppresses β-lactam resistant bacteria in a mixed microbial setting

Transformed E. coli were grown overnight at 37 °C on Luria Bertani Broth (Lennox) (LB) agar plates containing the appropriate antibiotics (50 μg/mL kanamycin and/or 100 μg/mL ampicillin). A single colony was used to inoculate 5 mL of liquid LB with the appropriate antibiotics and grown at 37 °C and 200 rpm for 20 h. Cells were harvested by centrifugation (4000 rcf, 5 min, rt), resuspended in 0.5 mL of ultrapure water, and DNA was isolated using Zymo Research Zyppy Plasmid Miniprep Kit. Purified DNA was sent to plasmidsaurus for whole-plasmid Oxford Nanopore sequencing.

.dna files of plasmid maps can be opened with SnapGene Viewer, a freely accessible software. <https://www.snapgene.com/snapgene-viewer> (accessed 2025-01-30)