Effect of Phoenixin-20 on gene expression in LPS and IFN-gamma-stimulated RAW264.7 cells under high-glucose conditions

Chronic inflammation driven by impaired macrophage plasticity is a hallmark of diabetic wound healing impairment. Phoenixin-20 (PNX-20), a conserved neuropeptide derived from the SMIM20 precursor, has been identified as a potent immunomodulatory factor involved in neuro-immune-endocrine regulation. However, its role in diabetic wound healing remains unclear. In this study, we investigated the transcriptomic effects of PNX-20 in RAW264.7 macrophages stimulated with LPS and IFN-gamma under high-glucose conditions, aiming to elucidate the molecular mechanisms underlying its anti-inflammatory activity. RNA sequencing was performed to identify genes and signaling pathways modulated by PNX-20 treatment. Our results revealed that PNX-20 reprograms macrophage polarization and suppresses inflammatory responses through activation of the GPR173/NDNF/p38 signaling axis. This dataset provides comprehensive transcriptional profiling of RAW264.7 cells under different inflammatory and treatment conditions, contributing to the understanding of neuropeptide-mediated immunoregulation in diabetic wound healing.