RNA seq study of fur mutant vs wt of A. salmonicida

The ferric uptake regulator (Fur) is a transcription factor and the main regulator of iron acquisition in prokaryotes. When bound to ferric iron, Fur recognizes its DNA binding site and generally executes its function by repressing transcription of its target genes. Due to its importance in virulence, the Fur regulon is well studied for several pathogenic model bacteria. In our previous work, we used computational predictions and microarray to gain insights into Fur-regulation in A. salmonicida, and have identified a number of genes and operons that appear to be under direct Fur-regulation. To provide an even more accurate and deeper global understanding of the Fur regulon we now generated an A. salmonicida fur knock-out strain and used RNA-sequencing to compare gene expression between the wild-type and fur null mutant strains.