A-to-I editing and adr mutant gene expression in C. elegans germlines

In this study, we performed high-throughput RNA-seq on young adult germline samples from wild-type and adr-2(-) C. elegans hermaphrodites. A-to-I editing sites were identified using SAILOR. Differential gene expression analysis was performed using DESeq2. Germlines were isolated from synchronized young adult C. elegans hermaphrodites raised at 20 degrees C. Strains used include N2 and BB20 (adr-2(ok735)). RNA was extracted and poly-A selection was performed prior to library preparation.