NK-92 amplicon sequencing of CIRSPR/Cas9 genome editing

NIAID Data Ecosystem2026-03-11 收录

CRISPR-Cas9基因编辑

sgRNA效率预测

数据链接：

https://www.ncbi.nlm.nih.gov/sra/SRP250619 数据链接链接失效反馈

官方服务：

资源简介：

To evaluate on-target and off-target efficiency of different sgRNAs

创建时间：

2020-05-21

Additional file 1: of Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: a multi-center evaluation

CRISPR-Cas9基因编辑

小鼠遗传模型分析

Table S1. The guide RNA and singlestranded Oligonucleotide DNA sequences, their concentrations and the length of genomic regions floxed (in bp) reportedÂ in this study. (XLSX 29 kb)

Figshare2024-02-15 更新80

Target-bound SpCas9 complex with TRAC full RNA guide

CRISPR-Cas9基因编辑

结构生物学

Target-bound SpCas9 complex with TRAC full RNA guide

NIAID Data Ecosystem50

U2OS cell line -SpCas9 inhibitor- Raw sequence reads

CRISPR-Cas9基因编辑

抑制剂筛选

To detect the cleavage activity of SpCas9 in U2OS after treat with small molecular inhibitor of SpCas9

NIAID Data Ecosystem30

CRISPR/Cas9 Editing of Bovine Mammary Epithelial Cells

CRISPR-Cas9基因编辑

泌乳基因功能研究

In vitro investigation of bovine lactation processes is limited by a lack of physiologically representative cell models. This deficiency is most evident through the minimal or absent expression of lac

NIAID Data Ecosystem60

Engineering Tripartite Gene Editing Machinery for Highly Efficient Non-Viral Targeted Genome Integration

CRISPR-Cas9基因编辑

非病毒靶向基因组整合

We developed Cas9 fusion proteins containing single-stranded DNA-binding proteins and a 20-amino acid motif with single-stranded DNA-binding function. These fusion proteins demonstrated higher efficie

NIAID Data Ecosystem40

NK-92 amplicon sequencing of CIRSPR/Cas9 genome editing

资源简介：

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