Zinc accumulation in Atriplex lentiformis is driven by plant genes and the soil microbiome

To investigate the effect of different levels of compost treatment on root gene expression of Atriplex lentiformis, we set up a greenhouse experiment with three treatments of 10% (TC10), 15 (TC15), and 20% (TC20) compost amended, metalliferrous mine tailings. Plants were harvested at ~11 weeks and root samples were flash frozen in liquid nitrogen for RNA-seq analysis. We then performed gene expression profiling analysis using data obtained from RNA-seq of 9 root samples from 3 different treatments. Gene expression changes across the root samples from the three treatments were estimated using the likelihood ratio test (LRT) in the DESeq2 analysis.