Genome editing-driven refinement of GPCR visualization in mouse models
收藏资源简介：
This dataset contains all supplementary materials associated with the manuscript:“Genome editing-driven refinement of GPCR visualization in mouse models” (Inoue YU et al., under review at Scientific Reports). The files include raw and processed data used in the figures and analyses reported in the paper, as well as step-by-step experimental protocols to facilitate reproducibility. Contents: In Cellulo GEF Assay Protocol.docx: Step-by-step protocol of in cellulo GEF assay. Raw Data_Fig. S1A-B.xlsx: Raw data used for Figures S1A and S1B. Raw Data_Fig. 3 & S1C-F.xlsx: Raw data used for Figures 3 and S1C-F. Input protein sequences for AlphaFold3 prediction.txt: Amino acid sequences used for AlphaFold3 structure prediction. Oxtr-3xHA.pse: Structural models of Oxtr-3xHA predicted by AlphaFold3. Oxtr-3xALFA.pse: Structural models of Oxtr-3xALFA predicted by AlphaFold3. Oxtr-smFP.pse: Structural models of Oxtr-smFP predicted by AlphaFold3. smFP.pse: Structural models of smFP predicted by AlphaFold3. Dataset_Fig. S1 & 3.xlsx: Processed datasets used for Figures S1 and 3. All data were generated in the laboratory of Ikuo Masuho (Sanford Research). Funding: NIH NIGMS R35GM156391 “Structure and Function Studies of GPCRome”. Correspondence: ikuo.masuho@sanfordresearch.org The dataset is made available under the CC-BY 4.0 license to promote transparency and reuse.
