Influence of L-lactate and low glucose concentrations on the metabolism and the toxin formation of Clostridioides difficile. Influence of L-lactate and low glucose concentrations on the metabolism and the toxin formation of Clostridioides difficile

Metabolomic and transcriptomic analysis of changes in the exponential and stationary phase of Clostridioides difficile after cultivation in casamino acids medium (reference) and supplemented with L-lactate or glucose and the connection to toxin production. Overall design: Clostridioides difficile 630∆erm (DSM28645) was grown in a casamino acids medium (reference) and supplemented with 2 g/L glucose (Glc) or 0.5 g/L L-lactate (Lac). Samples were taken at exponential growth (1/2 ODmax) and at the beginning of the stationary phase (ODmax).