Flow cytometric analysis of thymic epithelial cells from fetal thymic organ cultures
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http://flowrepository.org/id/FR-FCM-ZYD2
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资源简介：
Evaluate the abundance and proportion of thymic epithelial cells in fetal thymic organ cultures derived from embryonic day 14.5 thymic lobes
Notes:
Thymic lobes were isolated from E14.5 C57BL/6 (B6) embryos and cultured on Isopore membrane filters (Millipore) placed on Gelfoam sponge (Pfizer) floated in RPMI-1640 medium supplemented with 10% heat-inactivated fetal calf serum, 50 mM 2-mercaptoethanol, 2 mM L-glutamine, 100 U ml-1 penicillin / streptomycin (Life Technologies). For depletion of thymocytes, thymic lobes were cultured for 5 days with 1.35 mM 2’-deoxyguanosine (SIGMA). Thymic lobes were disaggregated with 0.1 % DNase I (Sigma) and 0.01% Liberase (Roche) prior to flow cytometry analysis.
创建时间：
2017-10-01
