Temperature dependence of mechanical and morphogenetic parameters.

*P≤0.05;
**P≤0.01:
Significant difference between 16 and 26°C for log transformed parameters.
1Estimated mean and confidence interval for log-transformed parameters were determined using ANOVA (mechanics and actin) and Tukey's honestly significant difference criterion, or T-tests (morphogenesis).
Values were then transformed back to a linear scale to provide estimates of the lower and upper bounds (LB, UB) on the ratio.
2Comparing 17° and 27°C. Ratios for the durations of actin contractions were 0.9 between 16.9°C and 21.3°C, and 1.5 between 21.3°C and 26.7°C.
To obtain confidence bounds here, we used ANOVA with temperature treatment as a categorical variable, and explant as a random factor; clutch was excluded because it was non-significant.