El-Desouky Ammar, Saskia A. Hogenhout, L. R. Nault (2011) CIL:25379, Maize mosaic virus, Peregrinus maidis. CIL. Dataset

Aggregates of maize mosaic virus (MMV, Rhabdoviridae) in cells of the anterior diverticulum of the insect vector Peregrinus maidis (planthopper, Hemiptera, Delphacidae). Antibodies to MMV identify aggregates (green/yellow fluorescence) that accumulate mainly around the nuclei (red) in cells of the anterior diverticulum. Accompanying image in this image group is unlabeled.

Methods: Dissected insect organs were fixed in paraformaldehyde, labeled with primary antibodies to MMV, and with secondary antibodies: Alexa Fluor-488 (green), and later stained with the nuclear stain propidium iodide (red). Whole mounts were examined with confocal laser- scanning microscope (Leica TCS SP).

Associated references:
Ammar, E.-D. and S. A. Hogenhout, S.A. (2008). A neurotropic route for Maize mosaic virus (Rhabdoviridae) in its planthopper vector Peregrinus maidis. Virus Research 131: 77-85.
Ammar, E.-D. and Nault, L.R. (1985). Assembly and accumulation sites of Maize mosaic virus in its planthopper vector. Intervirology 24: 33-41.

玉米花叶病毒（MMV，Rhabdoviridae科）在害虫传播者Peregrinus maidis（跳甲，半翅目，飞虱科）前肠细胞中的聚集。针对MMV的抗体可识别主要累积于细胞核周围（红色荧光）的聚集（绿色/黄色荧光）。本图像组中伴随的图像未标注。

方法：将昆虫器官解剖后，用多聚甲醛固定，并用针对MMV的初级抗体及二抗（Alexa Fluor-488，绿色）进行标记，随后用核染色剂碘化丙啶（红色）染色。使用共聚焦激光扫描显微镜（Leica TCS SP）进行整体观察。

相关参考文献：
Ammar, E.-D. and S. A. Hogenhout, S.A. (2008). 玉米花叶病毒（Rhabdoviridae科）在其跳甲传播者Peregrinus maidis中的神经毒性途径。病毒研究 131: 77-85。
Ammar, E.-D. and Nault, L.R. (1985). 玉米花叶病毒在其跳甲传播者中的组装和积累位点。病毒学杂志 24: 33-41。