Broth-cultured vs consolidation-phase P. mirabilis
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE17897
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Swarming motility by the urinary tract pathogen Proteus mirabilis has been a long-studied, but little understood phenomenon. On agar, a P. mirabilis colony grows outward in a bullseye pattern formed by consecutive waves of rapid swarming followed by consolidation into shorter cells. To examine differential gene expression in these growth phases, a microarray, constructed based on the completed genome sequence and annotation, was undertaken. RNA from 1) broth-cultured, or 2) consolidation-phase cells was extracted to assess transcription during each of these growth states. Five independent arrays (biological replicates) were each analyzed for broth-cultured vs consolidation-phase P. mirabilis.
创建时间:
2012-03-21



