Additional file 1 of Activity of Plasmodium vivax promoter elements in Plasmodium knowlesi, and a centromere-containing plasmid that expresses NanoLuc throughout the parasite life cycle

Additional file 1: Table S1. Oligonucleotide primers used in this work. Table S2. Transient transfection of Plasmodium parasites. Table S3. Plasmodium knowlesi growth and luminescence signal during selection using WR99210. Table S4. NanoLuc signal of in vitro cultivated transgenic blood-stage parasites. Table S5. Rhesus infections with transgenic luminescent P. knowlesi. Table S6. Number of oocysts detected in individual mosquitoes dissected 7 days after the feeds and Luminescence signals obtained (only midguts isolated from mosquitoes from Feed #2). Table S7. Total sporozoites isolated from 8 mosquitoes from each species, infected with transgenic bioluminescent P. knowlesi, 10 days after the feeds and Luminescence signals. Table S8. pvcen-pvhsp70-D-NanoLuc plasmid copy number variation in blood-stage parasites. Table S9. Half-maximum inhibitory concentration (IC50) measures of drug response. Table S10. Plasmodium knowlesi red blood cell invasion assays.附加文件1：表S1 本研究中使用的寡核苷酸引物（Oligonucleotide primers）。表S2 疟原虫（Plasmodium）的瞬时转染实验。表S3 使用WR99210进行筛选时诺氏疟原虫（Plasmodium knowlesi）的增殖情况与发光信号。表S4 体外培养的转基因血液阶段疟原虫的纳米荧光素酶（NanoLuc）信号。表S5 感染转基因发光型诺氏疟原虫的恒河猴感染实验。表S6 吸血后7天解剖的单个蚊虫体内检测到的卵囊数量及获取的发光信号（仅包含第2次饲喂组蚊虫分离的中肠样本）。表S7 吸血后10天，从每种感染转基因生物发光型诺氏疟原虫的蚊虫中选取8只，分离得到的总子孢子数量及发光信号。表S8 血液阶段疟原虫中pvcen-pvhsp70-D-NanoLuc质粒的拷贝数变异情况。表S9 药物反应的半数最大抑制浓度（IC50）测定结果。表S10 诺氏疟原虫的红细胞入侵实验。