LAP-induced monocyte chemotaxis is blocked by specific inhibitors of LAP-TSP-1 interactions.

Human monocytes (5×104/condition) were suspended in Gey's balanced salt solution and assayed for their chemotactic response to rhLAP. Recruitment was assayed by incubating monocytes in modified Boyden chambers for 90 minutes at 37°C and quantified by counting five high-powered fields of stained membranes in a blinded fashion and reported as fold-increase of LAP (10 pg/ml)-induced monocyte chemotaxis compared to monocyte chemotaxis induced by media alone. rhLAP at 10 pg/ml was chosen based on previous experiments that determined this dose induced maximal cellular recruitment. These data represent n = 3 (minimum) separate experiments expressed as the mean fold-change±SEM. Reported p values compare fold-change in monocyte chemotaxis induced by LAP without and with identified inhibitors.